Some facts

There are many approaches to distinguish chromosomal sub-regions in or by mFISH banding. Most important are MCB/ mband and RX-FISH.

mFISH banding was introduced, as mFISH methods using human wcp probes reach their limits, when exact breakpoint localization of translocations are required, or in case of intra-chromosomal rearrangements such as interstitial deletions or inversions. Thus, different approaches have been developed to overcome that limitations.

For review see:

• Liehr T, Heller A, Starke H, Claussen U (2002) Fluorescence in situ hybridization (FISH) banding methods - applications in research and diagnostic. Expert Review of Molecular Diagnostics 2:217-225.
•  Liehr T, Starke H, Weise A, Lehrer H, Claussen U (2004) Multicolor FISH probe sets and their applications. Histol Histopathol 19:229-237.
• Liehr T, Starke H, Heller A, Kosyakova N, Mrasek K, Gross M, Karst C, Steinhaeuser U, Hunstig F, Fickelscher I, Kuechler A, Trifonov V, Romanenko SA, Weise A (2006) Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding. Cytogenet Genome Res 114: 240-244.
• Liehr T, Gross M, Karst C, Glaser M, Mrasek K, Starke H, Weise A, Mkrtchyan H, Kuechler A (2006) FISH banding in tumor cytogenetics. Cancer Genet Cytogenet 164:88-89.
• Liehr T, Weise A, Hinreiner S, Mkrtchyan H, Mrasek K, Kosyakova N (2010) Characterization of chromosomal rearrangements using multicolor-banding (MCB/m-band). Methods Mol Biol 659:231-238.