Cross-species color banding (Rx-FISH)

The probes initially used are flow sorted chromosomes of two gibbon species (Hylobates concolor and H. syndactylus), which show both highly rearranged karyotypes with respect to the human one. Using this probe set the chromosomes #15, #18, #21, #22 and the sex chromosomes do not differentiate in segments [Müller et al., 1997]. Thus, chromosomes of another gibbon species (H. leucogenys) have been flow sorted, split in three portions, labeled with three different fluorochromes and the resulting probe set has been called Rx-FISH [Müller et al., 1998]. A resolution of 160 bands per haploid karyotype can be achieved, when combining the Rx-FISH probe set with 110 human-hamster somatic cell hybrids mentioned before [bar code section], split in 2 different pools and labeled with 2 additional fluorochromes [Müller and Wienberg, 2000].

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• Hills LV, Nouri S, Slater HR (2003) Pericentromeric euchromatin is conserved in minute human supernumerary chromosomes: a study using cross-species colour segmenting (RxFISH). Chromosome Res 11:359-363
  

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• Müller S, Wienberg J (2000) Advances in the development of chromosome bar codes: Integration of M-FISH and Rx-FISH technology. medgen 12: 474-477 (German).
  

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• Neusser M, Munch M, Anzenberger G, Muller S (2005) Investigation of marmoset hybrids (Cebuella pygmaea x Callithrix jacchus) and related Callitrichinae (Platyrrhini) by cross-species chromosome painting and comparative genomic hybridization. Cytogenet Genome Res 108: 191-196.
  

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• Teixeira MR, Micci F, Dietrich CU, Heim S (2000) Detailed genome-wide screening for inter- and intrachromosomal abnormalities by sequential G-banding and RxFISH color banding of the same metaphase cells. Cancer Genet Cytogenet 119: 94-101.
  

• Teixeira MR (2002) Combined classical and molecular cytogenetic analysis of cancer. Eur J Cancer 38: 1580-1584.
  

• Wienberg J (2005) Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution. Cytogenet Genome Res 108:139-160.
  

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• Zhao L, Hayes K, Glassman A (2000) Enhanced detection of chromosomal abnormalities with the use of RxFISH multicolor banding technique. Cancer Genet Cytogenet 118: 108-111.
  

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