Centromeric and single copy probes

mFISH methods can be performed using locus-specific single-copy (i.e. cosmids, P1-clones, BACs, YACs) - as mentioned above for the “chromosome bar code” . Apart from applications for special scientific approaches like the characterization of chromosomal sub-regions using mFISH on chromosome fibers (fiber-FISH) [Duell et al., 1997; Conti et al., 2007] locus-specific probes are used in some special mFISH approaches, mostly without, sometimes together with centromeric probes. Also centromeric probes can be applied seperately in mFISH.

• Duell T, Nielsen LB, Jones A, Young SG, Weier HU (1997) Construction of 
  two near-kilobase resolution restriction maps of the 5' regulatory 
  region of the human apolipoprotein B gene by quantitative DNA fiber 
  mapping (QDFM). Cytogenet Cell Genet 79: 64-70.
  

• Conti C, Herrick J, 
  Bensimon A (2007) Unscheduled DNA replication origin activation at 
  inserted HPV 18 sequences in a HPV-18/MYC amplicon. Genes Chromosomes 
  Cancer 46:724-734.