FISH

mFISH - CENTROMERIC AND SINGLE COPY PROBES

Basics and literature on multicolor fluorescence in situ hybridization application

 

mFISH using alphoid / centromeric probes

 

  • Brecevic L, Michel S, Starke H, Muller K, Kosyakova N, Mrasek K, Weise A, Liehr T (2006) Multicolor FISH used for the characterization of small supernumerary marker chromosomes (sSMC) in commercially available immortalized cell lines. Cytogenet Genome Res 114:319-324.

  • Gutiérrez-Mateo C, Benet J, Starke H, Oliver-Bonet M, Munné S, Liehr T, Navarro J (2005) Karyotyping of human oocytes by cenM-FISH, a new 24-colour centromere-specific technique. Hum Reprod 20:3395-3401.

  • Henegariu O, Bray-Ward P, Artan S, Vance GH, Qumsyieh M, Ward DC (2001) Small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). Lab Invest 81: 475-481.

  • Kitsiou-Tzeli S, Manolakos E, Lagou M, Kontodiou M, Kosyakova N, Ewers E, Weise A, Garas A, Orru S, Liehr T, Metaxotou A (2009) Characterization of a prenatally assessed de novo supernumerary minute ring chromosome 20 in a phenotypically normal male. Mol Cytogenet 2:1.

  • König K, Göhlert A, Liehr T, Loncarevic IF, Riemann I (2000a) Two-Photon Multicolor FISH: a versatile technique to detect specific sequences with in single DNA molecules in cells and tissues. Single Molecules 1: 41-51.

  • König K, Riemann I, Fischer P, Halbhuber KJ (2000b) Multiplex FISH and three-dimensional DNA imaging with near infrared femtosecond laser pulses. Histochem Cell Biol 114:337-345.

  • Langer S, Fauth C, Rocchi M, Murken J, Speicher M (2001) AcroM fluorescent in situ hybridization analyses of marker chromosomes. Hum Genet 109: 152-158.

  • Lian J, Yin Y, Oliver-Bonet M, Liehr T, Ko E, Turek P, Sun F, Martin RH (2008) Variation in crossover interference levels on individual chromosomes from human males. Hum Mol Genet 17:2583-2594.

  • Liehr T, Nietzel A, Rocchi M, Heller A, Starke H, Claussen U, v Eggeling F (2002) Centromere-specific multicolor-FISH (cenM-FISH) followed by analysis for uniparental disomy - a useful tool in prenatal diagnosis. In: Early prenatal diagnosis, fetal cells and DNA in the mother - present state and perpectives. The Karolinum Press, Prag, pp 293-300, ISBN: 80-246-0397-7.

  • Liehr T, Nietzel A, Starke H, Heller A, Weise A, Kuechler A, Senger G, Ebner S, Martin T, Stumm M, Wegner R, Tönnies H, Hoppe C, Claussen U, von Eggeling F (2003) Characterization of small marker chromosomes (SMC) by recently developed molecular cytogenetic approaches. J Ass Genet Techn 29: 5-10.

  • Liehr T, Mrasek K, Starke H, Claussen U, Schreiber G (2005) Unusual small supernumerary marker chromosome (sSMC) 9 in a Klinefelter patient. Cytogenet Genome Res 111: 179-181.

  • Liehr T, Brude E, Gillessen-Kaesbach G, Konig R, Mrasek K, von Eggeling F, Starke H (2005) Prader-Willi syndrome with a karyotype 47,XY,+min(15)(pter->q11.1:) and maternal UPD 15--case report plus review of similar cases. Eur J Med Genet 48: 175-181.

  • Moskovszky L, Szuhai K, Krenács T, Hogendoorn PC, Szendroi M, Benassi MS, Kopper L, Füle T, Sápi Z (2009) Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis. Genes Chromosomes Cancer 48:468-479.

  • Nietzel A, Rocchi M, Starke H, Heller A, Fiedler W, Wlodarska I, Loncarevic I, Beensen V, Claussen U, Liehr T (2001) A new multicolor-FISH approach for the characterization of marker chromosomes: Centromere-specific multicolor-FISH (cenM-FISH). Hum Genet108: 199-204. 

  • Oliver-Bonet M, Liehr T, Nietzel A, Heller A, Starke H, Claussen U, Codina-Pascual M, Pujol A, Abad C, Egozcue J, Navarro J, Benet J (2003) Karyotyping of human synaptonemal complexes by cenM-FISH. Eur J Hum Genet 11:879-83

  • Oliver-Bonet M, Benet J, Sun F, Navarro J, Abad C, Liehr T, Starke H, Greene C, Ko E, Martin RH (2005) Meiotic studies in two human reciprocal translocations and their association with spermatogenic failure. Hum Reprod 20:683-688.

  • Oliver-Bonet M, Benet J, Martin RH (2006) Studying meiosis: a review of FISH and M-FISH techniques used in the analysis of meiotic processes in humans. Cytogenet Genome Res 114:312-318.

  • Rubes J, Lowe X, Moore D, Perreault S, Slott V, Evenson D, Selevan SG, Wyrobek AJ (1998) Smoking cigarettes is associated with increased sperm disomy in teenage men. Fertil Steril 70: 715-723.

  • Starke H, Nietzel A, Weise A, Heller A, Mrasek K, Belitz B, Kelbova C, Volleth M, Albrecht B, Mitulla B, Trappe R, Bartels I, Adolph S, Dufke A, Singer S, Stumm M, Wegner RD, Seidel J, Schmidt A, Kuechler A, Schreyer I, Claussen U, von Eggeling F, Liehr T (2003) Small supernumerary marker chromosomes (SMCs): genotype-phenotype correlation and classification. Hum Genet 114:51-67.

  • Starke H, Mrasek K, Liehr T (2005) Three cases with enlarged acrocentric p-arms and two cases with cryptic partial trisomies. J Histochem Cytochem 53:359-360.

  • Sun F, Oliver-Bonet M, Liehr T, Starke H, Trpkov K, Ko E, Rademaker A, Martin RH (2005) Discontinuities and unsynapsed regions in meiotic chromosomes have a cis effect on meiotic recombination patterns in normal human males. Hum Mol Genet 14:3013-3018.

  • Sun F, Oliver-Bonet M, Liehr T, Starke H, Turek P, Ko E, Rademaker A, Martin RH (2006) Analysis of non-crossover bivalents in pachytene cells from 10 normal men. Hum Reprod 21:2335-2339.

  • Sun F, Oliver-Bonet M, Liehr T, Starke H, Turek P, Ko E, Rademaker A, Martin RH (2006) Variation in MLH1 distribution in recombination maps for individual chromosomes from human males. Hum Mol Genet 15:2376-2391.

  • Sun F, Oliver-Bonet M, Liehr T, Starke H, Ko E, Rademaker A, Martin RH (2007) Discontinuities and unsynapsed regions in meiotic chromosomes have a trans effect on meiotic recombination of some chromosomes in human males. Cytogenet Genome Res 119:27-32.

  • Sun F, Mikhaail-Philips M, Oliver-Bonet M, Ko E, Rademaker A, Turek P, Martin RH (2008) The relationship between meiotic recombination in human spermatocytes and aneuploidy in sperm. Hum Reprod 23:1691-1697.

  • Uroz L, Liehr T, Mrasek K, Templado C (2009) CenM-FISH on human spermatocyte I and II metaphases. Hum Reprod 24:2029-2033.

  • von Beust G, Sauter SM, Liehr T, Burfeind P, Bartels I, Starke H, von Eggeling F, Zoll B (2005) Molecular cytogenetic characterization of a de novo supernumerary ring chromosome 7 resulting in partial trisomy, tetrasomy, and hexasomy in a child with dysmorphic signs, congenital heart defect, and developmental delay. Am J Med Genet A 137: 59-64.

  • von Eggeling F, Hoppe C, Bartz U, Starke H, Houge G, Claussen U, Ernst G, Kotzot D, Liehr T (2002) Maternal uniparental disomy 12 in a healthy girl with a 47,XX,+der(12)(:p11-->q11:)/46,XX karyotype. J Med Genet 39: 519-521.

  • Yurov YB, Soloviev IV, Vorsanova SG, Marcais B, Roizes G, Lewis R (1996) High resolution multicolor fluorescence in situ hybridization using cyanine and fluorescein dyes: rapid chromosome identification by directly fluorescently labeled alphoid DNA probes. Hum Genet 97: 390-398.